TNF-α inhibition restrained IR-enhanced colorectal tumor growth in the liver. The mice were sacrificed at seven days after IR, and their livers were harvested to estimated tumor growth. (A) Tumor load (The arrows indicated tumors), (B) total tumor volume, mm3 = (long diameter × short diameter2)/2 (C) tumor number (per liver of each animal were counted). (D) H&E stained histopathological sections (magnification × 50) and (E) percentages of hepatic replacement area were shown. Data are presented as mean ± SEM. (n = 5; *, P < 0.05; * *, P < 0.01). The data represent three separate experiments.