Inhibition of TNF-α suppressed IR-induced liver injury. The mice of each group were sacrificed at indicated times after IR. H&E stained histopathological sections at (A) 180 min, (B) 360 min. (C) Percentages of necrotic area and (D) relative MPO activity of liver homogenate at 360 min were shown. Data are presented as mean ± SEM. (n = 5; *, P < 0.05; * *, P < 0.01). The data represent three separate experiments.