Inhibition of TNF-α reduced IR-induced liver mRNA expressions of IL-6, MMP9 and E-selectin. The liver of each mice were harvested at 360 min after IR and their mRNA expressions of (A) IL-6, (B) MMP9 and (C) E-selectin were detected by qPCR. Data are presented as mean ± SEM. (n = 5; *, P < 0.05; * *, P < 0.01). The data represent three separate experiments.