Figure 2

Role of the MDA epitope in oxLDL-induced human coronary artery endothelial cell (HCAEC) cytotoxicity. (A) HCAECs were incubated for 24 hours with or without oxLDL (100 μg/ml) in the presence or absence of anti-MDA (0.15 μg/ml), preimmune serum, or anti-apoB100 (0.15 μg/ml), as indicated; control cells were treated with PBS. Cells are shown under the view of an inverted microscope. (B) Percentage of trypan blue–positive cells expressed as the mean ± SEM (n = 3). **P < 0.01 vs control. ^†P < 0.05 vs oxLDL alone. MDA, malondialdehyde; oxLDL, oxidized low-density lipoprotein.