Figure 3

Effect of anti-MDA on oxLDL-regulated FGF2 expression. (A) Intracellular FGF2 protein concentrations in cultured human coronary artery endothelial cells (HCAECs) are shown, as determined with ELISA. Cells were incubated for 24 hours with or without oxLDL (100 μg/ml) in the presence or absence of anti-MDA (0.01, 0.05, 0.1, or 0.15 μg/ml). Values shown are the mean ± SEM (n = 3). *P < 0.05, **P < 0.01 vs the oxLDL-untreated PBS control. ^†P < 0.05 vs the oxLDL-treated PBS control. (B) FGF2 mRNA levels in HCAECs relative to those in the PBS-treated control, as determined by using RT-PCR. HCAECs were incubated for 24 hours with the indicated reagents, and total RNA was subjected to RT-PCR analysis by using specific primers for FGF2 and the gene encoding β-actin (internal control). The upper panel shows a gel representative of 3 separate experiments, and the lower panel shows the densitometric quantification. MDA, malondialdehyde; oxLDL, oxidized low-density lipoprotein.