Figure 4

Effects of signal transduction inhibitors on the oxLDL-induced reduction of cell viability, as determined by using the MTT assay in cultured human coronary artery endothelial cells (HCAECs). HCAECs were incubated for 24 hours with signaling pathway modifiers in the presence or absence of oxLDL (100 μg/ml) and anti-MDA (0.15 μg/ml), as indicated. The cell viability after each treatment was normalized to that of the PBS control (first black column). Values shown are mean ± SEM (n = 3). *P < 0.05 vs control (first black column); **P < 0.05 between the indicated pair. 5-aza-dC, 5-aza-deoxycytidine; Akt inh, Akt inhibitor; MDA, malondialdehyde; oxLDL, oxidized low-density lipoprotein; PTX, pertussis toxin.