Effects of anti-MDA and oxLDL on the transcriptional regulation of FGF2 in cultured human coronary artery endothelial cells (HCAECs). (A) A diagram showing the different FGF2 reporter gene constructs used. Different lengths of human FGF2 5′-flanking sequences were cloned into the pGL3-basic luciferase reporter vector. +1 indicates the transcription start site. (B) FGF2 promoter activity is shown, as determined by using a luciferase reporter gene assay. HCAECs were cotransfected with the indicated constructs and phRL-TK (internal control) and were incubated for 24 h with or without oxLDL (100 μg/ml) in the presence or absence of anti-MDA (0.15 μg/ml). Luciferase activities are expressed relative to those in cells transfected with pGL3-basic. Values shown are the mean ± SEM (n = 3-5). *P < 0.05 vs corresponding oxLDL-untreated controls. Luc, luciferase reporter gene; MDA, malondialdehyde; oxLDL, oxidized low-density lipoprotein.