Figure 7

FGF2 promoter analysis. (A) The CpG island sequence in the promoter region of human FGF2 is shown. The human FGF2 promoter contains 20 CpG sites, numbered 1-20. The primers used for DNA methylation sequencing are shown. (B) An illustration showing the methylation status of the CpG dinucleotides in human coronary artery endothelial cells treated for 24 hours with oxLDL (100 μg/ml) in the presence or absence of anti-MDA (0.15 μg/ml). Unmethylated cytosines are indicated by stalks. Methylated cytosines are indicated by stalks with square heads. MDA, malondialdehyde; oxLDL, oxidized low-density lipoprotein.