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Figure 1 | Journal of Biomedical Science

Figure 1

From: Protection of palmitic acid-mediated lipotoxicity by arachidonic acid via channeling of palmitic acid into triglycerides in C2C12

Figure 1

Lipotoxicity of saturated fatty PA as a function of concentration (A) and the effect of unsaturated fatty acids on PA-mediated lipotoxicity (B). Cells grown at 2*104 were treated with increasing concentrations of PA for 24 h. To examine the protective effects of unsaturated fatty acids on lipotoxicity of PA, cells were coincubated with 0. 5 mM PA and 62.5 μM unsaturated fatty acids indicated for 24 h. LDH release into media was assayed for the evaluation of lipotoxicity mediated by supplementation of fatty acids, and relative LDH release for each group was calculated from absorbance values being set sample completely lysed in Triton X-100 and culture medium left untreated to 100% and 0%, respectively.

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