Figure 3

The dose dependent impairment of insulin-stimulated glucose uptake in C2C12 treated with PA (A) and improvement of glucose uptake in response to insulin by AA (B). Differentiated myotubes C2C12 cells were exposed to increasing concentrations of PA or 0.5 mM PA along with either AA or ETYA at the concentration of 62.5 μM for 24 h. Subsequently, cells were rinsed and subjected to glucose uptake assay in response to 100 nM insulin in a buffer containing 10 μM 2-deoxyglucose. Glucose uptake was expressed in an increase of DPM/well in response to insulin stimulation versus basal glucose uptake. *p <0.05 versus basal glucose uptake.