Figure 5

Augmentation of PA-driven lipid droplets by AA loads in C2C12 cells. The driving potency of each fatty acids to produce lipid droplets during C2C12 culture was measured by Nile red staining of cells grown in media containing various doses of fatty acids-BSA complex (A). Also, images of lipid droplets within cytosol were taken when cells were overloaded with 0. 5 mM PA alone (left), 0.5 mM PA plus 62.5 μM AA (middle) or 0.5 mM PA plus 62.5 μM ETYA (right) (B). To quantitate stimulated incorporation of PA into lipid droplets by unsaturated fatty acids, PA in combination with unsaturated fatty acids at 62.5 μM were loaded into C2C12 cell culture and allowed to incubate for 24 h. Relative fluorescence of neutral droplets were measured following Nile-red staining and fixation of them (C). *p < 0.05 versus PA-treated group.