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Figure 6 | Journal of Biomedical Science

Figure 6

From: Protection of palmitic acid-mediated lipotoxicity by arachidonic acid via channeling of palmitic acid into triglycerides in C2C12

Figure 6

The incorporation of PA into AA-driven neutral lipid TG. Microscopic images showing localization of fluorescent PA analogue BODIPY FL C16 within lipid droplets of C2C12 cells exposed to AA (A) and lipid analyses extracted from C2C12 cells on TLC (B). To examine the trafficking of PA into AA-driven TG, the myotubes were supplemented with either 7.5 μM of fluorescent PA analogue 7.5 μM BODIPY FL C16 alone or 7.5 μM of BODIPY FL C16 plus 62.5 μM AA or 62.5 μM ETYA for 24 h. Cells were washed with PBS, fixed with 10% formaldehyde solution and then observed for the incorporation of PA analogue into AA-driven TG using fluorescence microscopy equipped with a B filter set. For lipid analysis, total lipids were extracted from cells treated under the same condition as in above. Lipids on TLC were developed and visualized as described in Methods. Lane 1 and 2 were, respectively, loaded with lipids extracted from cells left treated with BODIPY FL C16 or BODIPY FL C16 plus AA.

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