Figure 4
Effects of BBD on hypoxia-induced ROS generation, IL-1, IL-6 and PGE 2 productions. PC12 and BV-2 cells were treated with 1, 10, 20 μM of BBD for 30 min. BBD reduced the generation of ROS under hypoxia in PC12 (A) and BV-2 (B) cells. MDA of PC12 (C) and BV-2 (D) cells were induced by 30-min hypoxia and reduced by BBD. BV-2 cells were treated with 1, 10, 20 μM of BBD under hypoxia 30 min. BBD concentration-dependently reduced hypoxia-induced IL-1 (E), IL-6 (F) and PGE2(G) production from BV-2 cells. Data are expressed as mean ± SEM of 3 independent experiments in triplicate. *: P < 0.05 as compared to the hypoxia control, n = 3.