Expression of IAPs, NAIP, Survivin and caspase-3 in the Western Blot and Immunohistocehemical analysis. Top Panel: Representative Western Blot analysis of NAIP (A), survivin (B) and caspase-3 (C). The prostate is collected from Sham rats treated with vehicle and BPH rats treated with either vehicle or SeR (25 mg/Kg) or Se (3 mg/kg) or Ly (1 mg/kg) or Ser + Se + Ly *p < 0.01 vs Sham; #p < 0.05 vs BPH + vehicle; •p < 0.01 vs BPH + vehicle; §p < 0.001 vs BPH + vehicle. Bars represent the mean ± SEM of seven experiments. Bottom Panel: Immunhistochemical detection of Caspase-3. The prostate is collected from Sham rats treated with vehicle (D) and BPH rats treated with either vehicle (E) or SeR (25 mg/Kg) (F) or Se (3 mg/kg) (G) or Ly (1 mg/kg) (H) or SeR + Se + Ly (I). Immunohistochemistry for caspase-3 showed a negative reaction in sham (D). In BPH rats treated with vehicle (E), a focal cytoplasmatic positivity was documented in acini and stromal cells. A progressive enhancement of positivity was observed in BPH rats treated with SeR (F), Se (G), Ly and with the combination of SeR + Se + Ly (I), where it reached the highest expression. Original magnification ×400.