Skip to main content
Figure 1 | Journal of Biomedical Science

Figure 1

From: Impact of glucose levels on expression of hypha-associated secreted aspartyl proteinases in Candida albicans

Figure 1

Expression of Sap5 in spider medium and lower glucose level media. C. albicans SC5314 was inoculated into various media with initial density of OD600 = 1/ml. Cells were harvested after cultured at 37°C for 1 hr. The media components were precipitated by 10% TCA for Western blot analysis and the cells were fixed by 3.7% formaldehyde for morphology observation. (A) The secreted Sap5 (Sap5p) was detected in precipitate of spider medium by Western blotting using polyclonal anti-Sap6 antibody which can recognize Sap4 ~ 6 proteins. (B) The analysis of morphological proportion of C. albicans SC5314 that was cultured in panel (A) described condition. (C) The Sap5 was rapidly and highly expressed in low glucose concentration (0.1%) during yeast-to-hypha transition. FBS: fetal bovine serum. (D) The analysis of morphological proportion of C. albicans SC5314 that was cultured in panel (C) described condition.

Back to article page