Figure 2

Low glucose level enhances hyphae formation and provokes the expression of hypha-associated Sap proteins. (A) C. albicans SC5314 was cultured at 37°C for 1 hr in YP medium containing different concentration of glucose. More of secreted Sap5 was detected in medium with lower glucose concentration (0.1%). (B) The analysis of morphological proportion of C. albicans SC5314 that was cultured in panel (A) described condition. (C) Abundant Sap5 and trace of Sap6 proteins were detected in Modified Lee’s medium containing 0.1% glucose after 2 hr incubation at 37°C. (D) The expression of Sap5 and Sap6 was impaired in cph1-null strain and was abolished in efg1-null strain. (E) Exploration the high glucose level represses Sap5 expression during yeast-to-hypha transition. C. albicans SC5314 was cultured in YP medium containing 0.1% glucose at 37°C. Glucose level was re-added up to 2% at indicated incubation times and then still cultured at 37°C; the total incubation period was 90 min. Secreted Sap5 was detected by Western blot analysis.