Expression of PAR2 is increased in glioam cells. Total RNA and proteins were extracted from surgically removed glioma tissue (3 patients), the marginal normal tissue and U87 cells; the samples were analyzed by qRT-PCR and Western blotting. A, the bars indicate the mRNA levels of PAR2 (mean ± SD; *, p < 0.01, compared with normal tissue). B, the immune blots indicate the protein levels of PAR2. The data represent 3 separate experiments.