Activation of TLR2 suppresses Alix in T84 cells. T84 cells were stimulated with SEB in the culture for 48 h. A, the bars indicate the mRNA levels of Alix. B, the immune blots show the results of Alix RNAi. C, the immune blots indicate the protein contents of Alix. D, the bars indicate the summarized density of the immune blots of panel C. a, TLR2-null T84 cells; b, T84 cells were treated with control shRNA. The data of bars are presented as mean ± SD. *, p < 0.01, compared with dose “0” group. The data represent 3 separate experiments.