SEB increases transcellular permeability of T84 monolayers. T84 monolayers were stimulated with SEB (the doses were denoted on the x axis of A and B) in Transwells for 48 h. A, the bars indicate the TER of the T84 monolayers. B, the bars indicate the OVA levels in the Transwell basal chambers; the OVA was expressed as a percentage of the OVA amounts added to the Transwell apical chambers. C, the immune blots show the Alix over expression results. D, the immune blots indicate the Alix gene knockdown results. The data of bars are presented as mean ± SD. *, p < 0.01, compared with the SEB dose “0”. The data represent 3 separate experiments.