OVA from Transwell basal chambers preserves antigenicity. CD4+ CD25- T effector (Teff) cells (labeled with CFSE) and DCs were isolated from the DO11.10 mouse spleen. The Teff cells and DCs were cultured at a ratio of 5:1 (T cell:DC) in the supernatant collected from the Transwell basal chambers. Apart from adding OVA to the apical chambers, the additional treatment included supernatant alone (A), SEB (B), Alix-over expressing T84 monolayers treated with SEB (C). Three days later, the cells were analyzed by flow cytometry. A-C, the histograms indicate the Teff cell proliferation. D is a no staining control. E, the bars indicate the summarized data from the histograms, the labels of x axis are the same as those of histograms. The data of E are presented as mean ± SD. *, p < 0.01, compared with group A. The data represent 3 separate experiments.