Effects of ICI 182, 780 on dioscin-induced ALP activity, Lrp5 and β-catenin mRNA expressions and ratio of OPG/ RANKL mRNA in MC3T3-E1 cells. MC3T3-E1 cells were treated with vehicle and dioscin (1.0 μg/ml) in the presence or absence of ICI 182, 780 (ICI). (A) ALP activity. Cell lysates were used for ALP activity measurement by an ALP activity assay kit. Results were obtained from three independent experiments and were expressed as mean ± SD. (B) Lrp5 mRNA expression level. (C) β-catenin mRNA expression level. (D) OPG mRNA expression level. (E) RANKL mRNA expression level. (F) OPG/RANKL mRNA ratio. (G) β-catenin protein expression level. Results were obtained from three independent experiments and expressed as mean ± SD. *P < 0.05; **P < 0.01 vs control. # P < 0.05; ## P < 0.01 vs Dio group.