Effect of dioscin on the ratio of OPG/ RANKL mRNA in MC3T3-E1 cells. MC3T3-E1 cells were treated with vehicle, different concentrations of dioscin (0.25 μg/ml, 0.5 μg/ml and 1.0 μg/ml) or lovastatin (0.04 μM) for 72 h, and then the expressions of OPG (A) and RANKL (B) mRNA were examined by RT-PCR. And the ratio of OPG/ RANKL mRNA (C) was obtained. Results were obtained from three independent experiments and were expressed as mean ± SD. *P < 0.05; **P < 0.01 vs Control.