Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines

Table 1 Nucleotide sequences of primers used for real time PCR detection

*Gene*	*Primer sequence* *(5’* *to 3’)*
Lrp5	Forward: ctgccaggatcgctctgatg
Lrp5	Reverse: acactgttgcttgatgaggacacac
β-catenin	Forward: gccacaggattacaagaagc
β-catenin	Reverse: ccaccagagtgaaaagaacg
OPG	Forward: ttacctggagatcgaattctgcttg
OPG	Reverse: gtgctttcgatgaagtctcagctg
RANKL	Forward: gcagcatcgctctgttcctgta
RANKL	Reverse: cctgcaggagtcaggtagtgtgtc
GAPDH	Forward: gaccacagtccatgccatcac
GAPDH	Reverse: gctgttgaagtcgcaggagac

ISSN: 1423-0127