Differential PTER expression level in the MN mouse model. At the end of the experiment, kidney samples were collected for histopathological examination and immunoblot analysis. Representative sections from renal tissue in MN (A, B and C) and NC (D, E and F) groups were examined with PTER immunohistochemical stain, original magnifications: 100× (A and D), 200× (B and E) and 400× (C and F). In addition, (G) represented negative controls staining without primary antibody in NC section, 400X. Total protein lysate was extracted from the renal cortical region and separated in 10% SDS page by electrophoresis, and further blotting by anti-PTER and β-actin antibodies (H). The bar chart represents the semi-quantification level of the expression density (I) (PTER/β-actin). *, p < 0.05.