Figure 4

PTER protein was specifically expressed in response to HSA-induced in vitro albuminuria model. Immunoblot analysis for the expression of PTER at 24 and 48 h in different concentrations of HSA (0.1, 0.5 and 1 mM)-treated HK-2 cells were examined (A). Data represent one out of three experiments. The relative PTER protein levels at 24 (B) and 48 h (C) were then normalized to β-actin and the results were presented as mean ± SD. a, p < 0.05 in comparison with the control group. The expression of PTER at 24 and 48 h in 0.03 mM γ-globulin-treated HK-2 cells were examined (D). The PTER protein expression levels were then normalized to β-actin and the results were presented as mean ± SD (E). N.S, No significant difference was found.