Figure 5

UV-B irradiation differentially regulates RF induced expression of miR-203 in vivo and in vitro. (A) Cortical neuronal cells were subjected to OGD were treated with either RF (5 μM) or UV-B-RF (5 μM). RF significantly increased the expression of miR-203 compared to that of UV-B-RF (B) RF treatment or miR-203 overexpression significantly decreased the LDH secretion compared to that of UV-B-RF treatment. (C) Percentage c-Jun 3′ UTR luciferase activity assay of wild type and mutated c-Jun in cortical neuron pre-treated with vehicle control and miR-203. (D) c-Jun expression in cortical neuron subjected to OGD with pre-treatment of mock, Control and miR-203. The average of three independent experiments performed in triplicate. Statistical significance was analyzed by ANOVA. Values are the mean ± SEM. *p < .05 and **p < 0.01 vs. RF treated and control group.