Figure 6

UV-B irradiation modulates the miR-203/c-Jun signaling pathway for altering the neuroprotective effect of RF. (A) Immunoblotting analysis of c-Jun expression in cell lysates from SH-SY5Y cell line and primary cortical neurons subjected to OGD and cortical regions of the brain subjected to ischemic injury and pretreated with either vehicle (saline), RF (10 mg/kg i.p.), UV-B-RF(10 mg/kg, i.p.) or miR-203 mimic (200 nM/kg i.p.). Representative images of at least three independent experiments are shown. (B) Cortical neuronal cells were subjected to OGD and treatment with RF (5 μM), miR-203 mimic (5 μM) , SP600125 (20 μM), c-Jun shRNA (50 nM/ml) and UV-B-RF (5 μM) treatment. Cell viability of cortical neurons was measured using the CCK-8 kit. UV-B-RF had significantly lower cell viability compared to that of the treated groups. The average of three independent experiments performed in triplicate. Statistical significance was analyzed by ANOVA. Values are the mean ± SEM. *p < 0.05 and **p < 0.01 vs. RF treated group.