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Figure 5 | Journal of Biomedical Science

Figure 5

From: Specificity protein 1 is a novel target of 2, 4-bis (p-hydroxyphenyl)-2-butenal for the suppression of human oral squamous cell carcinoma cell growth

Figure 5

The effect of HPB242 on apoptosis of HN22 and HSC4 cells. HN22 (A) and HSC4 (B) cells were incubated with HPB242 (0, 5, 10 and 20 μg/ml) for 48 h. Fifty micrograms of cellular extract per lane was separated on an SDS-PAGE gel as described in Methods. Immunoblot detection of Bid, Cleaved-caspase3, and PARP, upregulation of Bax, downregulation of Bcl-xl in whole cell lysates from various concentration HPB242-treated HN22 and HSC4 cells for 48 hours. Equal loading of protein was confirmed by incubating the same membrane with anti-β-Actin antibody.

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Journal of Biomedical Science

ISSN: 1423-0127