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Figure 3 | Journal of Biomedical Science

Figure 3

From: Characterization of a new mouse p53 variant: loss-of-function and gain-of-function

Figure 3

The new p53 variant has significantly less transactivation activity. (A) HeLa cells were transiently transfected with 0.4 μg pM (Gal4DBD) and the indicated wild-type, new variant or R175H mutant pM–p53 along with the GK1 reporter gene (0.2 μg). The luciferase activities of the transfected cell extracts were determined. Numbers above the bars indicate the fold increase in activation compared with the pM alone. (B) HeLa cells were transiently transfected with the indicated amount of wild-type or new variant pM–p53 along with the GK1 reporter gene (0.2 μg). These data are the average of three experiments (mean ± S.D.; n = 3).

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