Figure 5
From: Characterization of a new mouse p53 variant: loss-of-function and gain-of-function
The functional role of the new p53 variant depends on the endogenous wild-type p53 abundance. p53-/- MEF (A) and HeLa (B) cells were transiently transfected with the indicated amount of pSG5.HA.p53, new variant or R175H mutant p53 along with the pG13-LUC reporter gene (0.2 μg). The luciferase activities of the transfected cell extracts were determined. These data are the average of three experiments (mean ± S.D.; n = 3).