Figure 8
From: Characterization of a new mouse p53 variant: loss-of-function and gain-of-function
The new p53 variant, not wild-type p53, complexes with the arginine methyltransferase CARM1 in HeLa cells. HeLa cells were transiently transfected with 0.5 μg of the indicated pSG5.HA.p53 or new p53 variant along with 0.5 μg of pSG5.HA.CARM1. After 36 h of transfection, cell lysates were immunoprecipitated using an antibody against the p53 C-terminal region and then separated using protein A/G beads. Supernatant (left panel) or protein-bound A/G beads (pellet, right panel) were subjected to immunoblotting analysis using an antibody against HA (3 F10). We observed a similar expression pattern in three independent experiments.