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Figure 3 | Journal of Biomedical Science

Figure 3

From: A transgenic approach to study argininosuccinate synthetase gene expression

Figure 3

EGFP expression during kidney development in the transgenic mice. (A) Study of EGFP fluorescence by fluorescence dissecting microscopy. Conditions were as described in the legends of Figure 2A. (B) Comparison of fluorescence patterns of the kidney among the mouse lines. Kidney frozen sections taken from 1- to 4-week transgenic mice and non-Tg controls were examined by fluorescence microscopy. Exposure time was 50 ms except for 3G of 1 ms. Scale bar, 200 μm. (C) Localization of EGFP fluorescence. Frozen sections of kidneys from 1-week old transgenic mouse 3J were examined by fluorescence microscopy. A region of enlarged image was selected (white box) and shown in the lower panel. In each panel: left, fluorescence image; middle, DAPI staining; right, fluorescence image merged with DAPI staining. Particular regions are distinguished by dotted lines: C: cortex; M: medulla; CL: cortical labyrinth; MR: medullary ray. The glomerulus is circled in yellow. (D) Immunohistochemical localization of EGFP and ASS. Paraffin sections of the kidney from 4 week-old transgenic mouse 3G were incubated with an EGFP antibody (upper panel) or ASS antibody (lower panel). The slides were incubated with the biotinylated secondary antibody coupled with streptavidin conjugated-HRP and was visualized by the application of chromogen DAB to produce brown color when the slides were counterstained with hematoxylin. PCT: proximal convoluted tubule; DCT: distal convoluted tubule; G: glomerulus; PE: parietal epithelium. Scale bar, 200 μm. (E) EGFP fluorescence patterns during kidney development of the transgenic mice. Mouse kidney was fixed and embedded in OCT compound. Serial frozen sections were examined by fluorescence microscopy. In each panel: top, EGFP fluorescence image, middle, DAPI staining, bottom, EGFP fluorescence image merged with DAPI staining. The developmental stages and mouse lines employed are as indicated. Areas of the cortex (C) and the medulla (M) are distinguished by dotted lines. Scale bar, 200 μm.

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