Figure 6
From: A transgenic approach to study argininosuccinate synthetase gene expression
Determination of relative abundances of EGFP and mouse Ass mRNAs during kidney development by S1 nuclease mapping. (A) Phosphorimage analysis of the protected fragments from S1 nuclease mapping of kidney RNA. Condition was as described in the legends to Figure 5. (B) EGFP and Ass mRNA profiles during kidney development. The intensities of hybridization signals of EGFP, Ass and Gapdh mRNAs were quantified by a phosphorimager where the levels of EGFP and Ass mRNAs were normalized to the level of the Gapdh mRNA. Relative abundances of EGFP mRNA (upper panel) and Ass mRNA (lower panel) during kidney development are plotted by taking the stage of the highest level as 100%. Standard deviation (solid line) is shown if analysis was performed at least 3 times. If performed twice, average with interval of two values is shown (dotted line).