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Figure 1 | Journal of Biomedical Science

Figure 1

From: Erratum to: CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes

Figure 1

The CIP4 FCH domain is important for neonatal rat ventricular myocyte hypertrophy. Neonatal rat ventricular myocytes were transfected with control or CIP4 siRNA and then infected with adenovirus expressing myc-tagged CIP4 WT or ΔFCH protein. Myocytes were stimulated with 10 μM PE for two days as indicated. A. CIP4 proteins were detected using a mouse anti-CIP4 antibody against human CIP4 aa 411–501. (Rat and human CIP4 are 92% identical.) B. Immunocytochemistry for α-actinin (green), ANF (red) and Hoechst (blue); bar = 20 μm. C. Cross-section area of myocytes. n = 7. D. Fraction of myocytes expressing ANF. n = 6. ANOVA (two-factor with replication): p-value (among the four CIP4 expression conditions) = 0.005 (C) and = 0.02 (D); p-value (±PE) < 10-6 for both B and C. Post-hoc testing: *p-values vs. CIP4 siRNA-transfected myocytes; p-values comparing myocytes cultured ± PE; p-values vs. myc-CIP4 WT expressing myocytes.

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