Figure 2

Intermittent hypoxia (IH) effects on protein phosphatase 2A (PP2A) and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. (A) PP2A mRNA levels were determined by real-time quantitative polymerase chain reaction (qPCR) and (B) PP2A protein levels were determined by Western blot in PC12 cells after 4 days of room air exposure (RA4, n = 7), 3–4 days of IH (IH3, n = 5; IH4, n = 5), and IH4 along with superoxide dismutase (IH4 + SOD, n = 6), 1,10-phenanthroline (IH4 + Phe, n = 5) or okadaic acid (IH4 + OKA, n = 5). (C) ERK1/2 phosphorylation levels in PC12 cells were determined by Western blotting after RA4, IH3, IH4, IH4 + SOD, IH4 + Phe, IH4 + OKA and (D) immunocytofluorescence (green) after RA4, IH4, IH4 along with cantharidin (IH4 + Can, n = 5) and nicotine (IH4 + Nicotine, n = 5). *p < 0.05 compared with RA4. ^#p < 0.05 compared with IH4. Values are means ± SEMs.