The effects of visfatin on MUC8 MUC5B expression in NCI-H292 cells. (A-D) Real time RT-PCR and ELISA showed that visfatin significantly increased MUC8 and MUC5B expression: visfatin-induced MUC8 expression was significantly stronger than visfatin-induced MUC5B expression, and MUC8 and MUC5B mRNA expression peaked at 8 h after treatment with visfatin (300 ng/mL). Images are representative of three separate experiments performed in triplicate. Bars indicate the mean ± S.D. of three independent experiments performed in triplicate. * P < 0.05 compared with zero value.