The role of ROS in visfatin-induced MUC8 and MUC5B expression in NCI-H292 cells. (A) Flow cytometry showed that visfatin (300 ng/mL) significantly induced ROS formation, especially after 2 h. (B) RT-PCR showed that both NAC and DPI significantly attenuated visfatin-induced MUC8 and MUC5B mRNA expression. Images are representative of three separate experiments performed in triplicate. Bars indicate the mean ± S.D. of three independent experiments performed in triplicate. *P < 0.05 compared with zero value. **P < 0.05 compared with visfatin alone.