Figure 5
From: Visfatin induces MUC8 and MUC5B expression via p38 MAPK/ROS/NF-κB in human airway epithelial cells
The role of ROS in visfatin-activated phosphorylation of p38 MAPK in NCI-H292 cells. (A) Western blot showed that both NAC and DPI did not attenuate visfatin-activated phosphorylation of p38 MAPK. (B) Flow cytometry showed that SB203580 significantly inhibited visfatin-induced ROS formation. Images are representative of three separate experiments performed in triplicate. Bars indicate the mean ± S.D. of three independent experiments performed in triplicate. *P < 0.05 compared with zero value. **P < 0.05 compared with visfatin alone.