Characterization of BM-MSC cultured from normal or ischemic rats. (A,B) round-shape colonies of fibroblastic like NormBM-MSC and IschBM-MSC, respectively. (C,D) Proliferative activities of NormBM-MSC and IschBM-MSC were stained with anti-BrdU labeled FITC (green) and Hoechst (blue) incorporation. Magnification: 200× (E) Ficoll-fractionated mononuclear cell numbers from bone marrows of normal or ischemic rats. n = 13 ~ 16; *P < 0.05 (F) Numbers of colony forming unit-fibroblast (CFU-f) from bone marrow cultures at 6 and 14 days in vitro. n = 4 per group; *p < 0.05 IschBM-MSC vs. NormBM-MSC (G) Quantitative cell proliferation of NormBM-MSC and IschBM-MSC.