Conditioned medium from NormBM-MSC or IschBM-MSC increased cortical neuronal survival by enhancing neuronal connection and decreasing LDH release. (A) Neuronal cells, non-treated control. (B) NormBM-MSC Cm-treated neuronal cells (C) IschBM-MSC Cm-treated neuronal cells (D) βIII tubulin positive signal, as percentage of total area, quantified by Image J, in cortical neuronal cells. (E) LDH release to medium in cortical neuronal cells. Three days after treatment, cortical cell cultures were immunostained with anti-βIII-tubulin, a neuronal marker and medium were collected for LDH assay. Data are presented as means ± SEM from 8–10 independent experiments done in duplicate. *, **P < 0.05, 0.01 Treatment versus control. Magnification 200× (A-C).