Figure 3From: Structural and functional characterization of MERS coronavirus papain-like proteaseFluorescence spectrum of MERS-CoV PLpro. The protein of 1 μM was dissolved in 50 mM phosphate pH 6.5 (closed circles) or 9 M urea (open circles) and excited with 280 nm UV light. The protein fluorescence emission was monitored from 300 to 400 nm at 25°C.Back to article page