Figure 4

The continuous size distribution change of MERS-CoV PL^pro. (A) Traces of absorbance at 280 nm of the enzyme in the 50 mM phosphate pH 6.5 during the SV experiment. The protein concentration was 1 mg/ml. For clarity, only every four scan is shown. The symbols represent experimental data and the lines are the results obtained after fitted to the Lamm equation using the SEDFIT program [23, 26]. (B) and (C) show the continuous c(s) and c(M) distribution of PL^pro, respectively. The residual bitmap of the raw data and the best-fit results are shown in the inset.