Table 1 Purification of MERS-CoV PLprofrom E. coli
From: Structural and functional characterization of MERS coronavirus papain-like protease
Step | Total protein (mg) | Total activity (Ua) | Specific activity (U/mg protein) | Purification (-fold) | Recovery (%) |
|---|---|---|---|---|---|
Cytoplasmic fraction | 426 | 340 | 0.80 | 1 | 100 |
Ni affinity chromatography | 64.4 | 221 | 3.43 | 4.3 | 65 |
Gel-filtration by S-100 column | 41.9 | 168 | 4.01 | 5.0 | 49.4 |