Figure 2From: Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cellsThe effect of bradykinin (Bra), S1P and PGE on the expression of endothelial differentiation markers CD31, vWF and eNOS in ASCs differentiated to endothelial-like cells. Real-Time PCR was performed to evaluate target gene expression. GAPDH was used to normalize target gene expression levels. A no treatment group was used as control to calculate relative expression between treatments groups. Left panel is for CD31, middle panel is for vWF and right panel is for eNOS. A total n = 6 individual ASC cell lines were tested.Back to article page