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Figure 5 | Journal of Biomedical Science

Figure 5

From: Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cells

Figure 5

The expression of S1P1 receptor protein in ASC-differentiated to endothelial-like cells. Panel A: Western-blot for S1P1 and β-actin in four cell lines of ASC-differentiated endothelial cells. Panel B: Immunostaining for S1P1 and β-actin in ASC-differentiated to endothelial cells. Red fluorescence is for S1P1, green fluorescence is for β-actin, blue fluorescence is DAPI for nuclei. White arrows show the membrane distribution of S1P1. Western-blot coupled to immunostaining confirmed the expression of S1P1 in ASC-differentiated to endothelial-like cells.

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