Figure 5From: Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cellsThe expression of S1P1 receptor protein in ASC-differentiated to endothelial-like cells. Panel A: Western-blot for S1P1 and β-actin in four cell lines of ASC-differentiated endothelial cells. Panel B: Immunostaining for S1P1 and β-actin in ASC-differentiated to endothelial cells. Red fluorescence is for S1P1, green fluorescence is for β-actin, blue fluorescence is DAPI for nuclei. White arrows show the membrane distribution of S1P1. Western-blot coupled to immunostaining confirmed the expression of S1P1 in ASC-differentiated to endothelial-like cells.Back to article page