Figure 5

NFκB binding activities of the SDF-1 promoter region induced by resistin induction. (A) NFκB p50 activation was determined by a TF ELISA assay for 2 h in TSGH 9201 cells . All bar graphs represent folds of control cells (CL), mean ± SEM. *P < 0.05 versus CL. (B) ChIP assay was performed for p50 by using p50 antibody. (C) NFκB p50 translocations in nuclei by immunofluorescent staining. TSGH-9201 cells treated with 50 nM resistin or 0.2% DMSO (as control) for 12 h were fixed, permeabilized, and stained with anti-p50, and then observed by phase contrast microscopy (left panels) and fluorescence microscopy. Arrows indicate change and nuclear location.