Figure 1

Involvement of MAPK activation in CAPE-induced apoptosis. (A) CAPE induced DNA fragmentation in C6 glioma cells. Cells were treated with or without CAPE (50 and 100 μM) for the indicated times. DNA was separated on a 1.8% agarose gel, stained with ethidium bromide, and photographed under ultraviolet light. (B) Effect of CAPE on the activation of MAPKs. Cells were cultured in medium containing CAPE (50 μM) for the indicated times. Following treatment, cells were harvested, and cell lysates were prepared for western blot analysis using anti-phospho-ERK, anti-phospho-JNK, anti-phospho-p38, anti-ERK, anti-JNK, anti-p38, and anti-actin antibodies. (C) Effect of MAPK inhibitors on CAPE-induced caspase 3 activation and PARP cleavage. C6 glioma cells were pretreated with MAPK inhibitors for 1 h and then treated with CAPE for 24 h. Cell extracts were assessed by western blot analysis using anti-PARP and anti-procaspase 3 antibodies. The bands were visualized with an ECL reagent and quantified by densitometry. The data is representative of two independent experiments. (D) Effect of MAPK inhibitors on CAPE-induced C6 cells viability. The data are shown as the percentage of the control group. Data are presented as the mean ± S.D. of three independent experiments. *P < 0.05, compared with control.