Figure 2

Effect of CAPE-induced NGF/p75NTR activation on apoptosis in C6 glioma cells. (A) Effect of CAPE on the expression of p75NTR, TrkA, and NGF in C6 glioma cells. Cells were treated with CAPE (50 μM) for the indicated times. Protein in the total cell lysate was detected with anti-p75NTR, anti-TrkA, anti-NGF, and anti-actin antibodies. Bands were visualized with an ECL reagent and quantified. (B) Inhibition of CAPE-induced caspase-3 and PARP expression by the anti-p75NTR antagonist peptide. C6 glioma cells were pretreated with the anti-p75NTR inhibitor (25 and 50 μM) for 1 h and then treated with CAPE (50 μM) for 24 h. Cell extracts were assessed by western blot analysis with anti-procaspase-3, anti-PARP, and anti-actin antibodies. Bands were visualized with an ECL reagent and quantified by densitometry. The data is representative of two independent experiments.