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Figure 3 | Journal of Biomedical Science

Figure 3

From: Activation of neutral-sphingomyelinase, MAPKs, and p75 NTR-mediating caffeic acid phenethyl ester–induced apoptosis in C6 glioma cells

Figure 3

The relationship between MAPKs and NGF/p75NTR after treatment with CAPE in C6 glioma cells. (A) Effect of MAPK inhibitors on CAPE-induced NGF and p75NTR expression. C6 glioma cells were pretreated with a JNK inhibitor (SP600125, 10 μM), p38 inhibitor (SB20358, 10 μM), or MEK/ERK inhibitor (PD98059, 10 μM) for 1 h and then treated with CAPE (50 μM) for 12 h. Cell extracts were assessed by western blot analysis using anti-NGF, anti-p75NTR, and anti-actin antibodies. (B) Effect of the anti-p75NTR antagonist on CAPE-induced MAPK activation. C6 glioma cells were pretreated with the anti-p75NTR antagonist peptide for 1 h and then treated with CAPE for the indicated times. Cells extracts were assessed by western blot analysis using anti-phospho-p38, anti-phospho-ERK, anti-phospho-JNK, anti-p38, anti-ERK, anti-JNK, and anti-actin antibodies. Bands were visualized with ECL reagent and quantified by densitometry. The data is representative of two independent experiments. *, apparent reduction in expression. (C) Effect of MAPK inhibitors on CAPE-inducedp38 activation. Whole cell lysates from CAPE-treated C6 cells by western blotting analysis using antibodies against the phosphorylated forms of p38 (Thr180/Tyr182) and anti-actin.

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