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Figure 5 | Journal of Biomedical Science

Figure 5

From: Activation of neutral-sphingomyelinase, MAPKs, and p75 NTR-mediating caffeic acid phenethyl ester–induced apoptosis in C6 glioma cells

Figure 5

Effect of N-SMase on CAPE-induced MAPK activation and NGF/p75NTR expression. (A) Inhibition of CAPE-induced phosphorylation of MAPKs by GW4869. C6 glioma cells were pretreated with a sphingomyelinase inhibitor (GW4869, 20 μM) for 1 h and then treated with CAPE (50 μM) for the indicated times. Cell extracts were assessed by western blot analysis with anti-phospho-p38, anti-p38, anti-phospho-ERK, anti-ERK, anti-phospho-JNK, anti-JNK, and anti-actin antibodies. Bands were visualized with ECL reagent and quantified by densitometry. The data is representative of two independent experiments. *, apparent reduction in expression. (B) Inhibition of CAPE-induced NGF and p75NTR expression by a sphingomyelinase inhibitor. C6 glioma cells were pretreated with a sphingomyelinase inhibitor (GW4869, 20 μM) for 1 h and then treated with CAPE (50 μM) for the indicated times. Cell extracts were assessed by western blot analysis with anti-NGF, anti-p75NTR, and anti-actin antibodies.

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